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Corning Life Sciences transwell inserts costar 3422
Transwell Inserts Costar 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+inserts+costar+3422/96+well+plates/pm40593006-152-0-3
Average 90 stars, based on 1 article reviews
transwell inserts costar 3422 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Membrane:

Article Title: Combined treatment with paclitaxel and suramin prevents the development of metastasis by inhibiting metastatic colonization of circulating tumor cells.
Article Snippet: Metastatic disease accounts for most deaths due to breast cancer and thus identification of novel ways to prevent this complication remains a key goal.. A frequently employed preclinical model of breast cancer metastasis relies on xenografted human MDA-MB-231 cells, since these reliably produce both soft tissue and osseous metastases when introduced into the arterial circulation of athymic mice.. Herein, we explored the ability of suramin (SA), an agent shown to antagonize the effects of various stromal cell-derived growth factors relevant to bone marrow colonization of tumor cells, administered both with and without paclitaxel (PTX), to inhibit the development of MDA-MB-231 metastases.

Article Title: Resveratrol modulates the malignant properties of cutaneous melanoma through changes in the activation and attenuation of the antiapoptotic protooncogenic protein Akt/PKB
Article Snippet: .. For migration assays, transwell inserts (Costar 3422, Corning Transwell polycarbonate membrane from Sigma Aldrich) were coated on the lower surface with a 5 mg/ml solution of fibronectin (Sigma) overnight at 41C. ..

other:

Article Title: Ubiquitin-Specific Protease 14 Negatively Regulates Toll-Like Receptor 4-Mediated Signaling and Autophagy Induction by Inhibiting Ubiquitination of TAK1-Binding Protein 2 and Beclin 1.
Article Snippet: immunofluorescence Microscopy Cells were grown on glass coverslips overnight, fixed with 4% paraformaldehyde (Sigma-Aldrich, P-6148), and treated with 0.2% Triton X-100 to permeabilize for 30 min on ice.

Migration:

Article Title: Fibrogenesis-driven tumor progression in clear cell renal cell carcinoma: prognostic, therapeutic implications and the dual role of neuropilin-1
Article Snippet: The medium was replaced with serum-free medium, and images were captured at 0 and 48 h using an inverted microscope (IX73, Olympus, Germany). .. Transwell experiment: Migration assays were conducted by seeding 786-O and Caki-1 cells, adjusted to 2 × 10 5 cells/mL, into the upper chambers of Transwell inserts (Costar 3422, Corning, USA) at 10,000 cells per well in serum-free medium. ..

Article Title: Fibrogenesis-driven tumor progression in clear cell renal cell carcinoma: prognostic, therapeutic implications and the dual role of neuropilin-1.
Article Snippet: The medium was replaced with serum-free medium, and images were captured at 0 and 48 h using an inverted microscope (IX73, Olympus, Germany). .. Transwell experiment: Migration assays were conducted by seeding 786-O and Caki-1 cells, adjusted to 2 × 105 cells/mL, into the upper chambers of Transwell inserts (Costar 3422, Corning, USA) at 10,000 cells per well in serum-free medium. ..

Article Title: Resveratrol modulates the malignant properties of cutaneous melanoma through changes in the activation and attenuation of the antiapoptotic protooncogenic protein Akt/PKB
Article Snippet: .. For migration assays, transwell inserts (Costar 3422, Corning Transwell polycarbonate membrane from Sigma Aldrich) were coated on the lower surface with a 5 mg/ml solution of fibronectin (Sigma) overnight at 41C. ..



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Corning Life Sciences transwell membrane filter inserts corning costar cat. no. 3422
KLF2 overexpression suppresses the metastasis of CRC cells. (A) Expression of KLF2 in HCoEpiC, FHC, SW480, and RKO cell lines was detected by western blot assay. (B) Western blotting assessment of KLF2 overexpression in SW480 and RKO cell lines. (C) The invasion activity of transfected SW480 and RKO cells was detected by <t>transwell</t> assay. Scale bar: 100 μm. (D) Statistical representation of the transwell assay shown in (C). (E) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (F) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (G) Statistical representation of wound healing assays shown in (E) and (F). * P < 0.05; ** P < 0.01.
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KLF2 overexpression suppresses the metastasis of CRC cells. (A) Expression of KLF2 in HCoEpiC, FHC, SW480, and RKO cell lines was detected by western blot assay. (B) Western blotting assessment of KLF2 overexpression in SW480 and RKO cell lines. (C) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (D) Statistical representation of the transwell assay shown in (C). (E) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (F) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (G) Statistical representation of wound healing assays shown in (E) and (F). * P < 0.05; ** P < 0.01.

Journal: The Journal of Pathology: Clinical Research

Article Title: KLF2 inhibits colorectal cancer progression and metastasis by inducing ferroptosis via the PI3K / AKT signaling pathway

doi: 10.1002/cjp2.325

Figure Lengend Snippet: KLF2 overexpression suppresses the metastasis of CRC cells. (A) Expression of KLF2 in HCoEpiC, FHC, SW480, and RKO cell lines was detected by western blot assay. (B) Western blotting assessment of KLF2 overexpression in SW480 and RKO cell lines. (C) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (D) Statistical representation of the transwell assay shown in (C). (E) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (F) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (G) Statistical representation of wound healing assays shown in (E) and (F). * P < 0.05; ** P < 0.01.

Article Snippet: Migration and invasion experiments were performed using Boyden chambers consisting of transwell membrane filter inserts (Corning Costar, cat. no. 3422, Life Sciences, New York, USA) covered in Matrigel.

Techniques: Over Expression, Expressing, Western Blot, Activity Assay, Transfection, Transwell Assay, Migration, Wound Healing Assay

Overexpression of KLF2 inhibits CRC metastasis by inducing ferroptosis via GPX4. (A) Transfected cells were treated with/without ferroptosis inhibitor Fer‐1 (2 μ m ) for 24 h and then GPX4 expression was detected by western blot assay. (B) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (C) Statistical representation of transwell assay shown in (B). (D) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (E) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (F) Statistical representation of transwell assay shown in (D) and (E). * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: The Journal of Pathology: Clinical Research

Article Title: KLF2 inhibits colorectal cancer progression and metastasis by inducing ferroptosis via the PI3K / AKT signaling pathway

doi: 10.1002/cjp2.325

Figure Lengend Snippet: Overexpression of KLF2 inhibits CRC metastasis by inducing ferroptosis via GPX4. (A) Transfected cells were treated with/without ferroptosis inhibitor Fer‐1 (2 μ m ) for 24 h and then GPX4 expression was detected by western blot assay. (B) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (C) Statistical representation of transwell assay shown in (B). (D) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (E) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (F) Statistical representation of transwell assay shown in (D) and (E). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Migration and invasion experiments were performed using Boyden chambers consisting of transwell membrane filter inserts (Corning Costar, cat. no. 3422, Life Sciences, New York, USA) covered in Matrigel.

Techniques: Over Expression, Transfection, Expressing, Western Blot, Activity Assay, Transwell Assay, Migration, Wound Healing Assay